The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling
نویسندگان
چکیده
منابع مشابه
RNA-Assisted Nuclear Transport of the Meiotic Regulator Mei2p in Fission Yeast
Fission yeast Mei2p is an RNA-binding protein required for both premeiotic DNA synthesis and meiosis I. Mei2p binds to a polyadenylated RNA molecule, meiRNA, loss of which blocks meiosis I. Mei2p forms a dot in meiotic prophase nuclei. Here, we show that meiRNA is required for the nuclear localization of Mei2p and is detectable in the dot. However, Mei2p carrying a nuclear localization signal c...
متن کامل14-3-3 Protein Interferes with the Binding of RNA to the Phosphorylated Form of Fission Yeast Meiotic Regulator Mei2p
The switch from mitosis to meiosis is controlled by the Pat1(Ran1) kinase-Mei2p system in Schizosaccharomyces pombe. Mei2p promotes both premeiotic DNA synthesis and meiosis I, and its RNA binding ability is essential for these two processes. Mei2p forms a dot structure in the nucleus prior to meiosis I, aided by a specific RNA species named "meiRNA". Pat1 kinase phosphorylates Mei2p on two pos...
متن کاملSupplementary Material 14-3-3 Protein Interferes with the Binding of RNA to the Phosphorylated Form of Fission Yeast Meiotic Regulator Mei2p
Yeast Strains, Genetic Procedures, and Media S. pombe strains used in this study are listed in Table S1. The nine. Each transformant was streaked on SC-Trp-Leu-His-Met containing various amounts of 3-amino-1,2,4-triazole and examined for original rad24 strain was obtained from A. Carr. General genetic procedures for S. pombe were according to Gutz et al. (1974) [S2]. their growth in the absence...
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The yeast SBF transcription factor is a heterodimer comprised of Swi4 and Swi6 that has a well defined role in cell cycle-specific transcription. SBF serves a second function in the transcriptional response to cell wall stress in which activated Mpk1 mitogen-activated protein kinase of the cell wall integrity signaling pathway forms a complex with Swi4, the DNA binding subunit of SBF, conferrin...
متن کاملNucleocytoplasmic Shuttling of Endocytic Proteins
Many cellular processes rely on the ordered assembly of macromolecular structures. Here, we uncover an unexpected link between two such processes, endocytosis and transcription. Many endocytic proteins, including eps15, epsin1, the clathrin assembly lymphoid myeloid leukemia (CALM), and alpha-adaptin, accumulate in the nucleus when nuclear export is inhibited. Endocytosis and nucleocytoplasmic ...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2001
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(01)02567-4